148STUDY OF THE PROTECTIVE EFFECT OF BONE MARROW-DERIVED MESENCHYMAL STROMAL CELLS AND THEIR CONDITIONED CULTURE MEDIA ON CONTRAST INDUCED NEPHROPATHY IN SPRAGUE DAWLEY RATSContrast-induced nephropathy (CIN) is a common cause of acute kidney injury (AKI), especially in cardiac patients undergoing coronary angiography. This study aimed to investigate the efficacy of conditioned stem cells (SCs) culture media and bone marrow-derived mesenchymal stromal cells (BM-MSCs) in preventing CIN in rats. 28 male Sprague Dawley (S.D.) rats were divided into 4 equal groups: -ve control, +ve control, SCs-conditioned culture media-treated and BM-MSCs-treated. CIN was induced in the latter 3 groups and in order to prevent it, these groups received an injection intravenously 48 hours prior to contrast medium (CM) injection; +ve control (0.5 ml 0.9% saline), conditioned SCs culture media treated (0.5 ml culture media not containing stem cells) and stem cell treated (0.5 ml culture media containing 3.5 x106BM-MSCs). All rats were sacrificed 48 hours after CM injection. CM injection resulted in marked renal dysfunction. Administration of SCs-conditionedculture media resulted in significant reduction of serum creatinine (Scr), urea and caspase-3, partial reduction in oxidative stress markers and insignificant affection of histopathological parameters. Administration of BM-MSCs obviously prevented renal injury as it resulted in significant reduction of Scr, urea and necrotic tubules with significant anti-apoptotic and antioxidant effects. BM-MSCs, but not conditioned medium can fully prevent CIN in S.D.rats.mohammednephro1983@yahoo.comResearch622016Bone marrow MSCs,caspase-3,conditioned culture media,contrast-induced nephropathyMohammed K Nassar,Yomna Khater,Mohammed A Sobh,Amira K El-Hawary,Nahla H Anber,Mohamed AM SobNephrology and Dialysis Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.,Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt,Nephrology and Dialysis Unit, Internal Medicine Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.,Pathology department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.,Fellow of Biochemistry, Emergency Hospital, Mansoura University, Mansoura 35516, Egypt,Experimental Biology, Faculty of Science, Urology & Nephrology Center, Mansoura University, Mansoura 35516, Egypt.