140OVICIDAL AND LARVICIDAL ACTIVITIES AGAINST ANOPHELES GAMBIAE, ANTIOXIDANT AND ANTIBACTERIAL PROPRIETIES OF AUCOUMEA KLAINEANA PIERRE, CANARIUM SCHWEINFURTHII ENGL AND DACRYODES EDULIS (G. DON) H. J. LAM ESSENTIAL OILS FROM GABONAs part of ongoing research on the ovicidal and larvicidal activities against Anopheles gambiae, antioxidant and antimicrobial proprieties of a mixture of essential oils extracted from Aucoumea klaineana Pierre, Canarium schweinfurthii Engl. and Dacryodes edulis (G. Don) H. J. Lam with an aim regard in contributing to the search for beneficial uses of the previous mentioned medicinal plants of Gabon, antioxidant activities were evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The essential oils were tested against 4 bacteria by using disc diffusion and micro-dilution methods. Based on the ovicidal efficacy and larvicidal activity tested by WHO in (2005), the standard protocol against 3rd and 4th instar larvae of field-collected mosquitoes vectors of human disease members of the Anopheles gambiae was revealed. The Antioxidant Activity Index (AAI) of essential oils ranged from 0.82 to 2.07 and could now be compared to AAI of vitamin C (11.32) and BHT (7.85) while those of combination ranged from 0.48 to 1.93. The essential oils exhibited a strong DPPH free radical scavenging action, IC50 values ranged from 48 to 78 µg/mL for essential oils and 20.37 to 82.61 µg/mL for the combined essential oils. Essential oils of A. klaineana, C. schweinfurthii and D. edulis, showed antimicrobial effects against all microorganisms tested. The FIC indices ranged from 0.11 to 7.13 for paired combinations of A. klaineana, C. schweinfurthii and D. edulis essential oils. All the paired combinations showed synergistic effects against Streptococcus pyogenes and Staphylococcus aureus. Combinations of A. klaineana with D. edulis or C. schweinfurthii had showed some antagonism effects on the inhibition of Bacillus cereus and E. coli. Only the combinations of A. klaineana with C. schweinfurthii had revealed some indifferent effects against Bacillus cereus. Somewhat, ovicidal and larvicidal activities were demonstrated. The A. klaineana, C. schweinfurthii and D. edulis essential oils exhibited also larvicidal activity. The median lethal concentration (LC50) of Anopheles gambiae larvae ranged between 9.94-22.98 µg/mL. The LC50 value of Anopheles gambiae eggs ranged between 22.08-67.64 µg/mL. Dacryodes edulis showed the highest activity against the Anopheles gambiae eggs and larvae. Over all the three plants, essential oils of Aucoumea klaineana had showed the lowest activity of Anopheles gambiae eggs and larvae. Larvae were more susceptible than eggs. The present study reveals the potential of ovicidal efficiency and larvicidal activity against Anopheles gambiae using the combination of Aucoumea klaineana, Canarium schweinfurthii and Dacryodesedulis essential oils to increase antioxidant and antimicrobial proprieties.obamengonga@gmail.comResearch612016 Ovicidal efficacy,larvicidal activity,Anopheles gambiae, antioxidant and antimicrobial proprieties,combination of essential oilsLouis-Clément Obame-Engonga,Joseph-Privat Ondo,Guy-Stéphane Padzys,Cheikna Zongo,Alfred S. TraoréLaboratoires de Substances Naturelles et de Synthèses Organométalliques (LASNSOM), Université des Sciences et Techniques de Masuku B.P. 943, Franceville, Gabon.,Laboratoire de Recherche en Biochimie (LAREBIO), Université des Sciences et Techniques de Masuku B.P. 769, Franceville, Gabon.,Laboratoire de Physiologie Animale: Electrophysiologie-Pharmacologie-URAB, Université des Sciences et Techniques de Masuku, BP 913, Franceville Gabon. ,Centre de Recherche en sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN); UFR / SVT; Université de Ouagadougou ; 03 BP 7021 Ouagadougou 03, Burkina Faso.,Centre de Recherche en sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN); UFR / SVT; Université de Ouagadougou ; 03 BP 7021 Ouagadougou 03, Burkina Faso.