278LEONOTIS NEPETIFOLIA ETHANOLIC EXTRACT EVALUATED FOR ITS ANTI-UROLITHIATIC ACTIVITY IN VITRODrugs that increase urination are known as diuretics. Acute oedema of the Jung, nephrotic oedema syndrome, arterial hypertension, and fluid retention diseases may be treated with these drugs, which act on the kidney to increase urine excretion. Minerals that are solid and non-metallic and adhere to the urinary tract are referred to as urolithiasis. Worldwide, there are millions of people suffering from urolithiasis. A kidney stone is formed when promoters and inhibitors in the kidney are imbalanced. In the world, 12% of the population develops renal stones, with men causing 70% to 80% of the cases, and women experiencing 47-60% of the cases. Supersaturation, nucleation, growth, aggregation, and retention of the molecules within the kidneys are some of the stages involved in this process. This study aimed to evaluate in-vitro antiurolithiatic activities of ethanolic extracts of Leonotis nepetifolia whole plants. The whole plant of Leonotis nepetifolia were collected from the roadside area of Aditya Group of Institutions. The freshly collected plant were cleaned from dirt, dried under shade and then coarsely powdered manually. The powder was macerated in ethanol for a period of 7 days and then subjected to hot percolation for 8 hours. Then the solution was filtered, concentrated and dried. Analysing the time course of turbidity changes in artificial urine due to calcium oxalate crystallization after adding sodium oxalate solution determined the effect of extract on calcium oxalate crystallization. Turbidity at 620nm was measured using a UV-VISIBLE spectrophotometer to study calcium oxalate precipitation at 37°C and pH 6.8. The use of this plant in drug development may be possible as a result of this research. The extract should, however, be studied in animal models of lithiasis to find out how it works. Since the observed activity of the plant extract is due to other phytochemicals in it, it is vital to further analyse and isolate the major active componentspallavi.srihari999@gmail.comResearch1312023Leonotis Nepetifolia, Plant Extract, Therapeutic Efficacy, Anti-Urolithiatic ActivityPallavi M,Muthyala Greeshma Rani,Pilli Sravani,Faruk Abdullah Khan,namul Hoque,Sanidul Islam KhondokarAssistant Professor, Nimra College of Pharmacy, Jupudi , Ibrahimpatnam, NTR District, Andhra Pradesh, India,Nimra College of Pharmacy, Jupudi, Ibrahimpatnam, NTR District, Andhra Pradesh, India,Nimra College of Pharmacy, Jupudi, Ibrahimpatnam, NTR District, Andhra Pradesh, India,Nimra College of Pharmacy, Jupudi, Ibrahimpatnam, NTR District, Andhra Pradesh, India,Nimra College of Pharmacy, Jupudi, Ibrahimpatnam, NTR District, Andhra Pradesh, India,Nimra College of Pharmacy, Jupudi, Ibrahimpatnam, NTR District, Andhra Pradesh, India