300INSIGHTS INTO CARDIOVASCULAR DEVELOPMENT: ZEBRAFISH LARVAL SCREENINGS AND GENETIC DETERMINANTSThrough phenotypic-driven larval screenings utilizing Zebrafish larvae, we have gained valuable insights into cardiovascular development. Identifying genetic determinants of physiological phenotypes poses challenges due to the lack of in vivo assays with relevant dynamic ranges. In this study, we visualized the cardiovascular systems of Zebrafish embryos using high-speed video microscopy, with validation conducted using Zebrafish cardiomyopathy models and NO modulation. Our findings revealed significant differences in aortic flow rate, aortic flow velocity, and ventricular size between wild-type and zebrafish models of dilated cardiomyopathy. Additionally, we evaluated the effects of isoproterenol, ouabain, and verapamil on heart function in zebrafish embryos. Notably, zebrafish and mammals exhibit similar physiological responses, supported by shared amino acid sequences. These results underscore the potential of zebrafish behavior quantification at a resolution and dynamic range comparable to mammals, facilitating the dissection of model organisms using molecular genetics and small moleculesIndora@gmail.comResearch1222022Zebrafish larvae, cardiovascular development, Phenotypic-driven screenings,Dilated cardiomyopathy, 5. Highspeed video microscopy.Dr Vipul IndoraAssociate Professor, Department of Physiology, Gouri Devi Institute of Medical Sciences & Hospital, Durgapur -713212, West Bengal