321SYNTHESIS AND EVALUATION OF SOME NOVEL THIOCHROME DERIVATIVES AS ANTI-MICROBIAL AGENTSThe substitution of electron releasing methyl or hydroxyl substituent either at 3rd or at 4th position of phenyl ring i.e. compound 23, 24 and 29 showed better antimicrobial activity while –NH2 substituent Containing Compound (compound 30) at 4th position of phenyl ring exhibited good activity. By comparing the antimicrobial activity of all the synthesized compounds, we conclude that electron withdrawing substituent’s at 3rd, 4th and 5thposition of phenyl ring enhanced the activity while same substituent’s at 2nd, 4th and 6th position resulted in slight decrease in activity. Presence of electron releasing substituent’s at 3rd and 4th position of phenyl ring showed enhanced biological activity. While compared with the anti-inflammatory activity results, we conclude that the presence of electron releasing or withdrawing group at 4th position of phenyl ring resulted in an enhanced activity. Also, the presence of electron withdrawing group at 3rd or 5th or both positions of phenyl ring enhances the activity. Similarly, analgesic activity results showed that the electron withdrawing groups at 3rd , 4th and 5th position and electron releasing substituent’s at 3rd and 4th position of phenyl ring enhances the analgesic activitysb9807964868@gmail.comResearch14220241, 3, 5- triazine, Syntheses, Anti-microbial Agents, Pyrazolidine-3, 5-DioneSamsher Bahadur Bind*, Yogesh Singh Thakur, Ketkee Mandawar, Nishi Prakash JainRKDF College of Pharmacy NH-12, Hoshangabad Road, Bhopal, Madhya Pradesh, 462026