349PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL EFFECT OF ETHANOLIC MANGIFERA INDICA LINN. LEAF EXTRACT AGAINST SALMONELLA TYPHITyphoid fever, caused by Salmonella typhi, continues to pose a major public health challenge in many developing countries due to poor sanitation and the increasing emergence of antibiotic resistance. This study aimed to evaluate the phytochemical composition and antibacterial activity of the ethanolic leaf extract of Mangifera indica Linn. against Salmonella typhi. Fresh leaves of M. indica were shade-dried, powdered, and subjected to extraction using 90% ethanol in a Soxhlet apparatus to obtain the crude extract. Preliminary phytochemical screening of the extract revealed the presence of biologically active constituents such as alkaloids, flavonoids, tannins, saponins, and phenolic compounds, which are known for their antimicrobial and antioxidant properties. The antibacterial activity of the extract was assessed using the disc diffusion method against S. typhi. The results demonstrated a concentration-dependent increase in antibacterial activity, with zones of inhibition ranging from 15 mm to 27 mm at extract volumes of 10 µl to 40 µl, respectively. The standard antibiotic gentamycin (20 µl) produced a zone of inhibition of 36 mm, serving as a positive control. Although the activity of the plant extract was lower than that of the standard drug, it exhibited notable inhibitory effects against the test organism. The observed antibacterial activity may be attributed to the synergistic action of the identified phytochemicals, particularly flavonoids and tannins, which are known to disrupt bacterial cell walls and interfere with metabolic processes. These findings suggest that M. indica leaf extract possesses significant antibacterial potential against S. typhi and may serve as a promising natural source for the development of alternative or adjunct antimicrobial agents in the management of typhoid fever rajeevgandhi01@gmail.comResearch1612026Mangifera indica, phytochemical analysis, antibacterial activity, Salmonella typhi, Disc diffusion methodRajeev Gandhi R¹*, Chandra S², Sharan V³, Siranjeevi S³, Sivaraman V³, Sneka R1Associate Professor, Department of Pharmacology, Sri Lakshminarayan College of Pharmacy, Dharmapuri District, Tamil Nadu – 636809, India ²Principal, Sri Lakshminarayan College of Pharmacy, Dharmapuri District, Tamil Nadu – 636809, India ³UG Scholar, Department of Pharmacology, Sri Lakshminarayan College of Pharmacy, Dharmapuri District, Tamil Nadu – 636809, India