352PHYTOCHEMICAL INVESTIGATION AND IN-VITRO ANTICANCER AND ANTIFUNGAL EVALUATION OF ALTERNANTHERA PARONYCHIOIDESMedicinal plants are an important source of novel bioactive compounds with therapeutic potential. Alternanthera paronychioides (Amaranthaceae) is traditionally used in folk medicine for the management of inflammatory conditions, infections, and wound healing; however, its anticancer and antifungal potential has not been comprehensively explored. The present study was designed to investigate the phytochemical composition of A. paronychioides and evaluate its in-vitro anticancer and antifungal activities. Plant material was extracted using suitable organic solvents, followed by qualitative phytochemical screening. In-vitro anticancer activity was assessed using standard cytotoxicity assays on selected human cancer cell lines, while antifungal activity was evaluated against pathogenic fungal strains using agar diffusion and broth dilution methods. The extracts exhibited dose-dependent cytotoxicity against cancer cell lines and significant antifungal activity, particularly against Candida and Aspergillus species. The observed biological activities are attributed to the presence of flavonoids, phenolic compounds, tannins, and saponins. The findings support the traditional use of A. paronychioides and highlight its potential as a natural source of anticancer and antifungal agentsrajeevgandhi01@gmail.comResearch1612026Alternanthera paronychioides, phytochemicals, in-vitro anticancer activity, antifungal activity, medicinal plantsRajeev Gandhi R, Gokul M, Gokulkumar G2, Gokulnath C, Gokulraj B, Gowtham V1Professor, Sri Lakshminarayan College of Pharmacy, Dharmapuri, Tamil Nadu, India. 2B. Pharm, Students, Sri Lakshminarayan College of Pharmacy, Dharmapuri Tamil Nadu, India.