82AUGMENTATION OF POTENTIAL IMMUNOMODULATORY AND ANTI-INFLAMMATORY EFFECTS OF DIAZEPAM WITH RESVERATROL ON DEXTRAN SULPHATE SODIUM MODEL OF COLITIS IN RATSUlcerative colitis (UC) is chronic inflammatory disorder, affecting mucosa and submucosa of the large colon with chronic ulceration, and its treatment is based on anti-inflammatory drugs which cause undesirable intolerable side effects, leading to a search for new and alternative therapies. Resveratrol and diazepam have immunomodulatory and antiinflammatory actions could be of value for UC. This study aims to investigate outcomes of concomitant use of diazepam with resveratrol on serum levels of IL-6, TNF-α and PGE2, corticosterone, colonic tissue levels of leucocytes count, myeloperoxidase (MPO) enzyme, malondialdehyde (MDA) and catalase (CAT) and superoxide dismutase (SOD) enzymes and with a histological analysis of colonic tissues with dextran sulphate sodium (DSS)-induced colitis from different rat groups in a comparison to sulfasalazine drug. 60 Wistar rats of either sex, 10 rats kept as normal group and remaining 50 rats were subjected to the induction of experimental colitis by dextran sulphate sodium 5%. Blood samples taken for serum assay of IL-6, TNF-α and PGE2, corticosterone. Colon strips tissues taken for histopathological evaluation and determining of concentration of leucocytes count, MPO, MDA, CAT, and SOD in colon tissues from different rat groups. Treatment with diazepam alone and resveratrol alone on colitis rat groups showed comparable results to that of colitis rat group treated with sulfasalazine while, concomitant use of both drugs resulted in significant reduction of serum levels of IL-6, TNF-α and PGE2, increase serum corticosterone and decrease colon tissue levels of leucocytes count, MPO, MDA, and with increased of CAT, and SOD and improved the histopathological architecture of colon; which are significantly superior to results of colitis rat group treated with sulfasalazine. Both diazepam and resvertrol, possessed obvious potential anti-inflammatory effects comparable to sulfasalazine, and their concomitant use showed augmentation of their potential immunomodualtory and anti-inflammatory actions, that supporting their potential use in management of human UC.ahmedpharma7@gmail.comResearch512015Ulcerative colitis,Inflammatory Bowel Disease,Proinflammatory Cytokines,Resveratrol,Diazepam,Antioxidant,ImmunomodulatoryAhmed A. A. BaderDepartment of Clinical Pharmacology, Faculty of Medicine, Menoufiya University, Egypt.