84ALDOSTERONE AS AN OXIDATIVE STRESS MARKER IN DOGAn investigation was carried out to determine the role of aldosterone as an oxidative stress marker in dogs. Sera were obtained to determine aldosterone, gamma glutamyl transferase (GGT) enzyme and electrolytes viz. sodium, potassium, chloride, calcium, phosphorus and magnesium during moderate and hot ambiences from healthy adult German shepherd dogs of either sex. Serum aldosterone levels were determined by radioimmunoassay. Results indicated significant (pï‚£0.05) increase in the levels of Serum Aldosterone, GGT, Sodium and Chloride during hot ambience as compared to moderate ambience. Mean values of serum potassium, calcium, phosphorus and magnesium showed reverse trend and the values were significantly (pï‚£0.05) lower during hot ambience as compared to moderate ambience. Since serum GGT is a well-established marker of oxidative stress, its increased levels indicated towards the development of oxidative stress in dogs due to hot ambience. Maximum percent change was observed in the mean value of serum GGT which was followed by change in serum aldosterone level in the present study. The present research work has assessed the association of aldosterone with the oxidative stress as well as with electrolytes. It can be concluded that extreme hot ambience produced the oxidative stress in the dogs. Interpretation of these variations will help to provide perceptive approach of various hidden mechanisms in the development of oxidative stress. Results clearly testified that in addition to disease conditions, stress due to changes in ambient temperature can also contribute in the alteration of aldosterone levels.nalinikataria@rediffmail.comResearch512015Aldosterone,Electrolytes,German Shepherd,Hot ambience,Oxidative stressAnubha Kataria,Ashish Josh,Anshika Kataria,Nalini Kataria Medical Practitioner, Bikaner-334001, Rajasthan, India,Teaching associate,Department of Veterinary Physiology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan, India,Final MBBS, Kasturba Medical College, Manipal, Karnataka, India.,Professor, Department of Veterinary Physiology, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner-334 001, Rajasthan, India. 3